Which is the most suitable part of the plant used to make virus free plants in micropropagation?

For raising a virus-free plant by the process of micropropagation various parts of the plants are used but the most suitable part is meristem as they divide at a very fast rate so the virus cannot reach there.

Simply so, which part is most suitable for virus free plants for micropropagation?

If the plant is infected by a virus, the apical and axillary meristem will remain free of virus. So, one can remove the meristem and grow it in-vitro to obtain virus-free plants.

Also Know, how do you make a plant virus free? A combination of meristem-tip culture and thermotherapy has been helpful in raising virus-free plants in many such cases. Shoot tips or nodal explants from infected plants are cultured in vitro after proper sterilization and subjected to heat therapy at 30–40^o C in a thermal chamber for varying periods.

Subsequently, one may also ask, which part of the plant is used for the propagation of virus free plants in tissue culture?

Meristem culture technique

Which one of the following tissue is most suitable for tissue culture to produce virus free plants of potato?

The meristem culture technique has been extended to a number of species to produce virus-free plants, and is now regularly used to produce virus-free plants in potato, dahlia, strawberry, carnation, chrysanthemum, orchids, etc.

Related Question Answers

Which part would you select as an explant to recover healthy plants from virus affected plants?

if you want to regenerate through calli formation then you have wide choices to select from depending on plants. Mostly for dicots, leaf tissue is a good choice.

Which milk is used in tissue culture?

Coconut milk

What is a callus plant?

Callus, In botany, soft tissue that forms over a wounded or cut plant surface, leading to healing. A callus arises from cells of the cambium. When a callus forms, some of its cells may organize into growing points, some of which in turn give rise to roots while others produce stems and leaves.

Why shoot tip is preferred for the production of virus free plants?

The shoot tip culture is the most used method to produce virus free plants. It is based in the assumption that pathogen concentration is not uniform throughout the infected plant. Cells uninvaded by pathogens are often found in the apical meristem of a rapidly elongating stem (Shabde-Moses and Murashige, 1979).

What is micropropagation in biology?

Micropropagation refers to the in vitro multiplication and/or regeneration of plant material under aseptic and controlled environmental conditions to produce thousands or millions of plants for transfer to the field.

Which one is required for obtaining virus free plants from virus infected plants?

To generate virus free plants, the apical meristem from the infected plant is collected and cultivated in an appropriate media, so that a new, complete and virus free plant can be obtained. It is due to the fact that viruses spread systematically in the whole plant except the meristems.

How do you treat plant viruses?

There are no cures for viral diseases such as mosaic once a plant is infected. As a result, every effort should be made to prevent the disease from entering your garden. Fungicides will NOT treat this viral disease. Plant resistant varieties when available or purchase transplants from a reputable source.

Do viruses attack plants?

Some viruses can infect plants when aphids and other insects tap into the phloem to feed. Such insect vectors can also pick up virus particles and carry them to new plant hosts. Other viruses infect plant cells through a wound site created by a leaf-munching insect such as a beetle.

Which method is used in virus indexing?

Polymerase chain reaction (PCR), reverse transcriptase PCR (RT-PCR), non-radioactive probe-based nucleic acid spot hybridisation (NASH) and enzyme-linked immune-sorbent assay (ELISA) based techniques were developed and validated for routine testing.

What is basic principle behind the thermotherapy for eradication of virus infection of plant?

Thermotherapy, simple in principle, consists in heat treatment of plant parts at temperature/time regimes that kill the conserved pathogen and that are only slightly injurious to the host. Heat is applied mainly by water, air, or vapor.

What is the necessity to produce virus free plants?

Production of virus-free plants is necessary to control viral diseases, import novel cultivars from other countries, exchange breeding materials between countries or regions and preserve plant germplasm. In vitro techniques represent the most successful approaches for virus eradication.

Why is meristem culture virus free?

A biotechnological alternative to obtain large quantities of healthy plants is the isolation of meristematic tissue, since this is generally free of viruses because its active cell division reduces differentiation of vascular tissues [5].

What does auxin stimulate in shoots?

In the shoots, auxin stimulates cell elongation and thus high concentrations of auxin promote growth (cells become larger) In the roots, auxin inhibits cell elongation and thus high concentrations of auxin limit growth (cells become relatively smaller)

What is crop resistance to yellow mosaic virus?

In mung bean, resistance to yellow mosaic virus and powdery mildew were induced by mutations.

Are embryos rescue?

Embryo rescue is one of the earliest and successful forms of in-vitro culture techniques that is used to assist in the development of plant embryos that might not survive to become viable plants. This technique nurtures the immature or weak embryo, thus allowing it the chance to survive.

Which type of water is available to plants?

Capillary water

Does plant grafting work?

Budding and grafting may increase the productivity of certain horticultural crops because they make it possible to do the following things: Change varieties or cultivars. An older established orchard of fruiting trees may become obsolete as newer varieties or cultivars are developed.

Why is it important to have virus free seed stock?

Disease-causing organisms infecting seed are viruses, fungi and bac- teria. Preventing transmission of these organisms is important in pro- ducing disease-free seed. means during germination could a virus in the seed coats infect the seedlings (33, 34).

Which plants are suitable for tissue culture?

Plants important to developing countries that have been grown in tissue culture are oil palm, plantain, pine, banana, date, eggplant, jojoba, pineapple, rubber tree, cassava, yam, sweet potato, and tomato. This application is the most commonly applied form of traditional biotechnology in Africa.

Who is the father of plant tissue culture?

Haberlandt

How can haploid plants be produced?

Haploid plants can be produced from immature pollen or microspores (male gametophytic cells). The pollen can be extracted by pressing and squeezing the anthers with a glass rod against the sides of a beaker.

Which plant part can be used to raise healthy plants from diseased plants?

So the correct option is 'meristem culture'. Was this answer helpful?

Which meristem is used in tissue culture?

1 Meristem Culture. Meristem culture is used for shoot apical meristem culture in vitro. Meristem culture was developed by Morel and Martin in 1952 for rivers eliminating from Dahlia [5].

What is root tip culture?

What is the Meaning of Root Culture? Root culture can be defined as the culture of excised radical tips of aseptically germinated seeds in a liquid medium where they are induced to grow independently under controlled conditions.